Vanderbilt University

Michael P. Stone, Ph.D.

Richard N. Armstrong Ph.D.Professor of Chemistry

Ph.D., University of California, Irvine, 1981 (Biophysical Chemistry)

The research in this lab is aimed at understanding how mutagenic chemicals perturb the structure of DNA and how such perturbations may play a role in mutagenesis. Oligodeoxynucleotides modified at specific sites allow adduct-induced conformational changes in DNA to be characterized in well-defined systems. A variety of optical and magnetic resonance spectroscopy techniques are utilized, with a major emphasis on NMR spectroscopy, to probe the solution conformation and molecular dynamics of modified oligodeoxynucleotides.

Recent Publications

Wang F, Elmquist CE, Stover JS, Rizzo CJ, Stone MP. DNA sequence modulates the conformation of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline in the recognition sequence of the NarI restriction enzyme. Biochemistry. 2007 46:8498-516.

Elmquist CE, Wang F, Stover JS, Stone MP, Rizzo CJ. Conformational differences of the C8-deoxyguanosine adduct of 2-amino-3-methylimidazo[4,5-f]quinoline (IQ) within the NarI recognition sequence. Chem Res Toxicol. 2007 20:445-54.

Cho YJ, Kozekov ID, Harris TM, Rizzo CJ, Stone MP. Stereochemistry modulates the stability of reduced interstrand cross-links arising from R- and S-alpha-CH3-gamma-OH-1,N2-propano-2'-deoxyguanosine in the 5'-CpG-3' DNA sequence. Biochemistry. 2007 46:2608-21.

Xu W, Merritt WK, Nechev LV, Harris TM, Harris CM, Lloyd RS, Stone MP. Structure of the 1,4-Bis(2'-deoxyadenosin-N(6)-yl)-2S,3S-butanediol intrastrand DNA cross-link arising from butadiene diepoxide in the human N-ras codon 61 sequence. Chem Res Toxicol. 2007 20:187-98.

Brown KL, Deng JZ, Iyer RS, Iyer LG, Voehler MW, Stone MP, Harris CM, Harris TM. Unraveling the aflatoxin-FAPY conundrum: structural basis for differential replicative processing of isomeric forms of the formamidopyrimidine-type DNA adduct of aflatoxin B1. J Am Chem Soc. 2006 128:15188-99.

Gold B, Marky LM, Stone MP, Williams LD. A review of the role of the sequence-dependent electrostatic landscape in DNA alkylation patterns. Chem Res Toxicol. 2006 19:1402-14. Review.

Voehler MW, Collier G, Young JK, Stone MP, Germann MW. Performance of cryogenic probes as a function of ionic strength and sample tube geometry. J Magn Reson. 2006 183:102-9.

Cho YJ, Wang H, Kozekov ID, Kozekova A, Kurtz AJ, Jacob J, Voehler M, Smith J, Harris TM, Rizzo CJ, Lloyd RS, Stone MP. Orientation of the crotonaldehyde-derived N2-[3-Oxo-1(S)-methyl-propyl]-dG DNA adduct hinders interstrand cross-link formation in the 5'-CpG-3' sequence. Chem Res Toxicol. 2006 19:1019-29.

Wang F, DeMuro NE, Elmquist CE, Stover JS, Rizzo CJ, Stone MP. Base-displaced intercalated structure of the food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline in the recognition sequence of the NarI restriction enzyme, a hotspot for -2 bp deletions. J Am Chem Soc. 2006 128:10085-95.

Cho YJ, Wang H, Kozekov ID, Kurtz AJ, Jacob J, Voehler M, Smith J, Harris TM, Lloyd RS, Rizzo CJ, Stone MP. Stereospecific formation of interstrand carbinolamine DNA cross-links by crotonaldehyde- and acetaldehyde-derived alpha-CH3-gamma-OH-1,N2-propano-2'-deoxyguanosine adducts in the 5'-CpG-3' sequence. Chem Res Toxicol. 2006 19:195-208.