Richard M. Caprioli, Ph.D.
Stanley Cohen Professor of Biochemistry, Chemistry, and Pharmacology
Director of the Mass Spectrometry Research Center
Ph.D., Columbia University, 1969 (Biochemistry)
The research interests of this laboratory are aimed at the investigation of biological processes involving the synthesis, modification, biological activity, storage, and degradation of peptides and proteins using modern mass spectrometric methods of analysis to follow molecular events. In recent years, the focus of the work has been the study of disease processes and the biochemical events involved in the metabolism of peptides (primarily in the brain and gut of mammals), encompassing the enzymatic breakdown of these peptides, their production from peptide and protein precursors, and the disruption of these processes by certain xenobiotics. Modern mass spectrometry technology, including liquid chromatography/electrospray/tandem mass spectrometry (LC/ESI/MS/MS) and matrix-assisted laser desorption ionization (MALDI) mass spectrometry are key technologies used for discovery and identification of proteins in both normal and diseased or compromised tissue. Special emphasis is placed on mass spectrometry imaging technology, a new method that involves molecular mapping of tissue through the production of ion images obtained from the analysis of mammalian tissue slices by MALDI mass spectrometry. These protein data can then be correlated with clinical effects and outcomes, serving in both a diagnostic and prognostic role.
Recent Publications
McLean JA, Ridenour WB, Caprioli RM. Profiling and imaging of tissues by imaging ion mobility-mass spectrometry. J Mass Spectrom. 2007 42:1099-105.
Suzuki K, Yu X, Chaurand P, Araki Y, Lareyre JJ, Caprioli RM, Orgebin-Crist MC, Matusik RJ. Epididymis-specific lipocalin promoters. Asian J Androl. 2007 9:515-21.
Norris JL, Cornett DS, Mobley JA, Andersson M, Seeley EH, Chaurand P, Caprioli RM. Processing MALDI mass spectra to improve mass spectral direct tissue analysis. Int J Mass Spectrom. 2007 260:212-221.
Raj JU, Aliferis C, Caprioli RM, Cowley AW Jr, Davies PF, Duncan MW, Erle DJ, Erzurum SC, Finn PW, Ischiropoulos H, Kaminski N, Kleeberger SR, Leikauf GD, Loyd JE, Martin TR, Matalon S, Moore JH, Quackenbush J, Sabo-Attwood T, Shapiro SD, Schnitzer JE, Schwartz DA, Schwiebert LM, Sheppard D, Ware LB, Weiss ST, Whitsett JA, Wurfel MM, Matthay MA. Genomics and proteomics of lung disease: conference summary. Am J Physiol Lung Cell Mol Physiol. 2007 293:L45-51.
Massion PP, Caprioli RM. Proteomic strategies for the characterization and the early detection of lung cancer. J Thorac Oncol. 2006 1:1027-39. Review.
Chaurand P, Schriver KE, Caprioli RM. Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections. J Mass Spectrom. 2007 42:476-89.
Hamblin M, Friedman DB, Hill S, Caprioli RM, Smith HM, Hill MF. Alterations in the diabetic myocardial proteome coupled with increased myocardial oxidative stress underlies diabetic cardiomyopathy. J Mol Cell Cardiol. 2007 42:884-95.
Groseclose MR, Andersson M, Hardesty WM, Caprioli RM. Identification of proteins directly from tissue: in situ tryptic digestions coupled with imaging mass spectrometry. J Mass Spectrom. 2007 42:254-62.
M'Koma AE, Blum DL, Norris JL, Koyama T, Billheimer D, Motley S, Ghiassi M, Ferdowsi N, Bhowmick I, Chang SS, Fowke JH, Caprioli RM, Bhowmick NA. Detection of pre-neoplastic and neoplastic prostate disease by MALDI profiling of urine. Biochem Biophys Res Commun. 2007 353:829-34.
Reyzer ML, Caprioli RM. MALDI-MS-based imaging of small molecules and proteins in tissues. Curr Opin Chem Biol. 2007 11:29-35.
Chaurand P, Norris JL, Cornett DS, Mobley JA, Caprioli RM. New developments in profiling and imaging of proteins from tissue sections by MALDI mass spectrometry. J Proteome Res. 2006 5:2889-900. Review.
Friedman DB, Wang SE, Whitwell CW, Caprioli RM, Arteaga CL. Multivariable difference gel electrophoresis and mass spectrometry: a case study on transforming growth factor-beta and ERBB2 signaling. Mol Cell Proteomics. 2007 6:150-69.
Khatib-Shahidi S, Andersson M, Herman JL, Gillespie TA, Caprioli RM. Direct molecular analysis of whole-body animal tissue sections by imaging MALDI mass spectrometry. Anal Chem. 2006 78:6448-56.
Johnson MD, Floyd JL, Caprioli RM. Proteomics in diagnostic neuropathology. J Neuropathol Exp Neurol. 2006 65:837-45. Review.
Amann JM, Chaurand P, Gonzalez A, Mobley JA, Massion PP, Carbone DP, Caprioli RM. Selective profiling of proteins in lung cancer cells from fine-needle aspirates by matrix assisted laser desorption ionization time-of-flight mass spectrometry. Clin Cancer Res. 2006 12:5142-50.
Cornett DS, Mobley JA, Dias EC, Andersson M, Arteaga CL, Sanders ME, Caprioli RM. A novel histology-directed strategy for MALDI-MS tissue profiling that improves throughput and cellular specificity in human breast cancer. Mol Cell Proteomics. 2006 5:1975-83.
Chaurand P, Cornett DS, Caprioli RM. Molecular imaging of thin mammalian tissue sections by mass spectrometry. Curr Opin Biotechnol. 2006 17:431-6.
Meistermann H, Norris JL, Aerni HR, Cornett DS, Friedlein A, Erskine AR, Augustin A, De Vera Mudry MC, Ruepp S, Suter L, Langen H, Caprioli RM, Ducret A. Biomarker discovery by imaging mass spectrometry: transthyretin is a biomarker for gentamicin-induced nephrotoxicity in rat. Mol Cell Proteomics. 2006 5:1876-86.
Luka Z, Ham AJ, Norris JL, Yeo EJ, Yermalitsky V, Glenn B, Caprioli RM, Liebler DC, Wagner C. Identification of phosphorylation sites in glycine N-methyltransferase from rat liver. Protein Sci. 2006 15:785-94.
Skold K, Svensson M, Nilsson A, Zhang X, Nydahl K, Caprioli RM, Svenningsson P, Andren PE. Decreased striatal levels of PEP-19 following MPTP lesion in the mouse. J Proteome Res. 2006 5:262-9.
Aerni HR, Cornett DS, Caprioli RM. Automated acoustic matrix deposition for MALDI sample preparation. Anal Chem. 2006 78:827-34.
Suzuki K, Yu X, Chaurand P, Araki Y, Lareyre JJ, Caprioli RM, Matusik RJ, Orgebin-Crist MC. Epididymis-specific promoter-driven gene targeting: a transcription factor which regulates epididymis-specific gene expression. Mol Cell Endocrinol. 2006 250:184-9.